The Morphology of Bull Spermatozoa
نویسنده
چکیده
It has been known since the nineteenth century that the microscopical structure of living cells may be modified by fixation and by post-mortem or sub-mortem changes (Hardy, 1899), but inadequate attention seems to have been paid to factors which may injure spermatozoa before fixation and microscopical examination. One such factor is the effect of sudden cooling. If semen is exposed to a sudden fall in temperature there is an irreversible decline in the activity of the spermatozoa (Chang & Walton, 1940), and this reduction in activity is associated with an increase in the number of dead, i.e. eosinophil cells (Lasley, Easley & McKenzie, 1942). This phenomenon of 'temperature shock' is encountered in bull semen cooled abruptly by pipetting semen at 30 C. into an aqueous solution of nigrosin and eosin at 20 C. (Hancock, 1951). The depressed rate of respiration and fructolysis of temperature-shocked samples can be correlated with the increase in the proportion of eosinophil cells (Bishop & Hancock,
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تاریخ انتشار 2005